O8060-13

Osteocalcin (Bone-Gla-Protein, BGP)

Size

£442.00

SKU:: O8060-13
Purity:: Purified by Ion Exchange chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Native bovine bone osteocalcin Extinction Coefficient: E 1%1cm, 280nm=14.0
Clone:: 8.H.13
Format:: Supplied as a liquid in 50% glycerol, H2O.
Extra Details:: Osteocalcin is single-chain (MW 5800) vitamin K-dependent protein produced by osteoblasts and found in high concentrations in bone. Applications: Suitable for use in RIA, ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution ELISA: 100-1000ug/ml. Coat plate with Osteocalcin at 5-10ug/ml. Western Blot: 10ug/ml Immunohistochemistry: 10-15ug/ml Note: Assays require 1-2mM CaCl2. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.