Organic Anion Transporter K1 (OAT-K1)

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£767.00

SKU:: O7799-01A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 14 AA Peptide sequence near the cytoplasmic, C-terminus of rat OAT-K1
Format:: supplied as liquid in PBS, pH 7.4 and 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Extra Details:: Introduction Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues. OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). A kidney-specific anion transporter termed OAT-K1 (rat 669 aa) has been cloned from rat kidney. OAT-K1 mRNA is specifically expressed in brush-borer membrane of the proximal straight tubules. Rat OAT-K1 (669 aa) shows 72% identity with rat liver oatp. It mediates basolateral uptake of anionic methotrexate but not PAH, taurocholate, and prostaglandin E2. OAT-K2 (498 aa), very similar to OAT-K1 (91% identity), has also been cloned from rat kidney. It also has 62-65% homology with oatp1-3 family of proteins. OAT-K2 is predominantly expressed in convoluted tubules, proximal straight tubules, and cortical collecting ducts. OAT-K2 mediates uptake of hydrophobic anions such as taurocholate, methotrexate, folate, and prostaglandin E2 suggesting that OAT-K2 participate in epithelial transport of hydrophobic anionic compound in the kidney. Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blot:1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL technique. ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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