Orexin 2 Receptor (Hypocretin 2 Receptor) (Not for Export EU)

Size

£704.00

SKU:: O7505-12
Purity:: Serum
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: 19 aa peptide near the C-terminus, cytoplasmic domain. Coupled to KLH.
Format:: Supplied as a liquid in PBS, 40% glycerol.
Extra Details:: Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (1-2). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is 130 amino acid long peptide with a putative 33-AA secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (1-2). These neuropeptides bind and activate two closely related Orexin receptors--G-protein coupled receptors (GPCRs) OX1R and OX2R. Rat and human OX2R are 460 aa, and 444 aa, respectively (1-2) Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.(see published refs) ELISA: 1:10,000-1:100,000 using 50-100ng of O7505-05 control peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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