O7502
Orexin Prepro (Hypocretin Prepro) (Not for Export EU)
Size
£704.00
- SKU:
- O7502
- Purity:
- Neat serum
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide, 17aa, corresponding to the carboxy terminus of murine prepro-Orexin. The sequence is not found in Orexin A or Orexin B.
- Format:
- Supplied as a lyophilized powder with 0.05% sodium azide.
- Extra Details:
- Prepro-orexin is 130 amino acid long peptide with a putative 33aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis. These neuropeptides bind and activate two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R.. Orexins (Orexin A and Orexin B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus. Orexin A and Orexin B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:100,000; Use 50-100ng of control peptide/well. Western Blot: 1-1000-1:5000l using ECL. Optimal dilution determined by the researcher. Control Peptide: O7502-10 Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice