O7502-05
Orexin Prepro (Hypocretin Prepro)
Size
£754.00
- SKU:
- O7502-05
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 17aa near the C-terminus of mouse prepro-Orexin, conjugated to KLH. Species Sequence Homology: rat; 82%, human; 70%.
- Format:
- Supplied as a lyopohilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
- Extra Details:
- Prepro-orexin is 130 amino acid long peptide with a putative 33 AA secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis. These neuropeptides bind and activate two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R.. Orexins (Orexin A and Orexin B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus. Orexin A and Orexin B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Applications: Suitable for use in ELISA. Other potential application, though not tested is Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:1000 using 50-100ng of control peptide/well. Optimal dilution determined by the researcher. Control Peptide: O7502-10: Orexin Prepro, Control Peptide (Hypocretin Prepro) Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice