O7501
Orexin B (Hypocretin B) (Not for Export EU)
Size
£704.00
- SKU:
- O7501
- Purity:
- Neat serum
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide, 13aa, amino terminus of murine Orexin B (KLH coupled).
- Format:
- Supplied as a lyophilized powder with 0.05% sodlium azide.
- Extra Details:
- Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (1). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is 130 amino acid long peptide with a putative 33 AA secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (1). These neuropeptides bind and activate two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:100,000 Using 50-100ng of control peptide/well. Western Blot: 1:1000-1:5000 Optimal dilution determined by the researcher. Control Peptide: O7501-10 Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice