Orexin B (Hypocretin B)

Size

£891.00

SKU:: O7501-01
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 13aa peptide mapping near the N-terminus of mouse Orexin-B. The immunogen peptide has 92% homology with rat and human Orexin-B.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Extra Details:: Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (1). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (1,2). These neuropeptides bind and activate two closely related Orexin receptors—G protein coupled receptors (GPCRs) OX1R and OX2R. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml using Chemiluminescence technique ELISA: 1:10,000-100,000 Immunohistochemistry: Not tested. It is recommended that the antibody be tried at 2-20ug/ml. Other applications not tested. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice