Orexin A (Orexin-A, Hypocretin (Orexin) Neuropeptide Precursor, Hypocretin 1, Hypocretin-1, HCRT1, Hypocretin A, Hypocretin, HCRT, Orexin, OX, PPORX, PPOX) (Not for Export EU)

Size

£735.00

SKU:: O7500-06B
Application:: IHC-Fr
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa14-33, CRLYELLHGAGNHAAGILTL to bovine Orexin A (KLH).
Format:: Supplied as a lyophilized powder. No preservative added. Reconstitute with 100ul sterile ddH2O. Centrifuge to remove any insoluble material.
Extra Details:: Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (1). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130aa peptide with a putative 33aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (1). These neuropeptides bind and activate two closely related Orexin receptors—G protein coupled receptors (GPCRs) OX1R and OX2R. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:1000-1:2000. Shows intense labeling of Orexin-A neurons with low background. Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice