O7044
ORAI1 (Transmembrane Protein 142A, TMEM142A, Calcium Release-Activated Calcium Channel Protein 1)
Size
£655.00
- SKU:
- O7044
- Purity:
- Purified by peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide correspoding to 18aa from near the N-terminus of human ORAI1.
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels (1 for review). ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry (2). It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor (3). ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. Applications: Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Immunohistochemistry: 10ug/ml Immunofluorescence: 20ug/ml Optimal dilutions to be determined by the researcher. Positive Control: WB: Human ovary tissue lysate IHC: Human spleen tissue Control Peptide: O7044-01: ORAI1, Human, Control Peptide (Transmembrane Protein 142A, TMEM142A, Calcium Release-Activated Calcium Channel Protein 1) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice