ORAI1, CT (Transmembrane Protein 142A, TMEM142A, Calcium Release-Activated Calcium Channel Protein 1)

Size

£655.00

SKU:: O7044-03
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 16aa from near the C-terminus of human ORAI1. Epitope: within the last 50aa. Species Sequence Homology: rat and chicken
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: ORAI1, also known as Transmembrane protein 142A (TMEM142A), is a 32.7 kD multi-pass membrane protein belonging to the ORAI family. Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI1 is a four- transmembrane spanning protein that is an essential component of CRAC. ORAI1 functions as a highly selective Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Defective ORAI1 in humans is the cause of one form of hereditary severe combined immune deficiency (SCID), which results in ablated T-cell Ca2+ entry. Applications: Suitable for use in Western Blot, ELISA, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml. Immunohistochemistry (paraffin): 2.5ug/ml. Optimal dilution to be determined by researcher. Recommended Positive Control: Human Ovary Total Protein Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice