ORAI1, CT (Transmembrane Protein 142A, TMEM142A, Calcium Release-Activated Calcium Channel Protein 1) (MaxLight 490)

Size

£947.00

SKU:: O7044-03-ML490
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 16aa from near the C-terminus of human ORAI1. Epitope: within the last 50aa. Species Sequence Homology: rat and chicken
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Extra Details:: MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. ORAI1, also known as Transmembrane protein 142A (TMEM142A), is a 32.7 kD multi-pass membrane protein belonging to the ORAI family. Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI1 is a four- transmembrane spanning protein that is an essential component of CRAC. ORAI1 functions as a highly selective Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Defective ORAI1 in humans is the cause of one form of hereditary severe combined immune deficiency (SCID), which results in ablated T-cell Ca2+ entry. Applications: Suitable for use in Western Blot, FLISA, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: paraffin Optimal dilution to be determined by researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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