O7034-50
Opticin (Oculoglycan)
Size
£767.00
- SKU:
- O7034-50
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Ala20-Thr332 from human Opticin, expressed in NS0 cells.
- Clone:
- 7H137
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Opticin (oculoglycan) is a member of the small leucine-rich proteoglycan (SLRP) family with homology to class III extracellular matrix family members epiphycan and mimecan. It forms a 90kD non-covalent dimer that is a component of vitreous collagen fibrils in the eye and is also expressed in the retina, iris, ligaments and skin. Mature human opticin shares 73% and 65% aa identity with mouse and rat, respectively. Mutations of opticin have been associated with age-related macular degeneration. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Using a colorimetric detection system, the detection limit is ~25ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. ELISA (Direct): 0.5-1ug/ml The detection limit is ~2ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice