O7030-51
Opioid Receptor, mu, phosphorylated (Ser377) (MOR1)
Size
£639.00
- SKU:
- O7030-51
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide corresponding to residues around Ser377 of human mu-Opioid Receptor. Sequence is homologous to Ser375 of mouse. MW: 70-90kD.
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Extra Details:
- The mu-opioid receptor (MOR) belongs to the superfamily of G-protein-coupled receptors. MOR mediates the analgesic and rewarding effects of morphine and other opiates as well as the actions of several endogenous opioid peptides. Upon binding to its ligands, this Gi-coupled receptor inactivates adenylyl cyclase and activates a variety of G-beta-gamma-dependent pathways including the MAPK and the PI3K/Akt cascades. Trafficking of these receptors to and from the plasma membrane and their desensitization play a significant role in morphine tolerance. As with other GPCRs, these processes are modulated by phosphorylation at diverse sites within intracellular domain. Among other sites, agonist-specific phosphorylation of serine 375 in mouse (serine 377 in human) MOR is essential for its internalization. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (IF-F): 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice