O6350-01F1
Omi, HtrA2 (HtrA Serine Peptidase 2, High Temperature Requirement Protein A2, HtrA-like Serine Protease, Omi Stress-regulated Endoprotease, PARK13, Protease Serine 25, PRSS25, Serine Protease 25, Serine Protease HtrA2 Mitochondrial, Serine Protease Omi) (Biotin)
Size
£685.00
- SKU:
- O6350-01F1
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide containing amino acids 335-350 of human Omi/HtrA2.
- Clone:
- 196C429
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Biotin.
- Extra Details:
- The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity (1-3). Recently, a serine protease has been isolated, which is released from the mitochondria upon induction of apoptosis by apoptotic stimuli. This protein called Omi/HtrA2 is a mitochondrial protein that binds to IAP (4). Like Smac/DAIBLO, the mature Omi protein contains a conserved IAP-binding motif (AVPS) at its N terminus. The deregulated expression of Omi in the cytoplasm of mammalian cells induces apoptosis indicating that Omi could participate in the mitochondrial apoptotic pathway. Positive Control: Jurkat or HL-60 cell lysate can also be used as a positive control. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 2-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice