O6151-01B

Oligodendrocyte Marker O4

Size

£800.00

SKU:: O6151-01B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: White matter of corpus callosum from bovine brain.
Clone:: 10K50
Format:: Supplied as a lyophilized powder from PBS, containing carbohydrates. Reconsittute with steile PBS.
Extra Details:: Oligodendrocytes are myelinating cells in the central nervous system (CNS) that form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O4 is an antigen on the surface of oligodendrocyte progenitors. It has been commonly used as the earliest recognized marker specific for the oligodendroglial lineage. Applications: Suitable for use in Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells (Rat differentiated cortical stem cells) Immunocytochemistry: 1-10ug/ml Optimal dilutions to be determined by the researcher. Hybridoma: Fusion of a mouse myeloma with B cells obtained from a mouse immunized with white matter of corpus callosum from bovine brain. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.