O5700-15
OGG1 (8-Oxoguanine DNA Glycosylase) (Not for Export EU)
Size
£704.00
- SKU:
- O5700-15
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- An 18 aa peptide close to the N-terminus of mouse OGG1 (KLH coupled)
- Format:
- Supplied as a liquid in PBS, containing 40% glycerol and 0.05% sodium azide.
- Extra Details:
- Mouse 8-Oxoguanine DNA Glycosylase 1 (mOGG1; 345aa) is involved in the excision of 7, 8-dihydro-8-oxoguanine produced as a result of DNA damage. This gene has been localized on human chromosome 3p25. Several forms of hOGG protein have been cloned differing in lengths (351-424aa) (refs 1). 8-Oxoguanine- DNA glyosylase (OGG1) is a DNA repair protein that is primarily responsible for the repair of 8-oxoguanine (8-oxo-G). It possesses both DNA glycosylase and AP (apurinic) lyase activity and is a functional analog of the bacteria Fpg protein. OGG1 releases 8- oxoguanine (8-oxo-G) from mutated DNA and nicks the DNA strand. Although its glycosidase activity is very efficient, its lyase activity is weak and is highest for substrates having a C as the opposite base. OGG1 is a b-lyase generating an -unsaturated sugar moiety at the 3’-terminus. Murine OGG1 is a 345 amino acid protein of 38.8kD. It displays 84% identity to the human OGG1. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:50,000 using 50-100ng control peptide per well. Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice