O5700-01

Ogg1 (8-Oxoguanine DNA Glycosylase)

Size

£685.00

SKU:: O5700-01
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to aa1-100 from human Ogg1. Species Sequence Homology: rabbit and porcine
Format:: Supplied as a liquid in PBS, 0.05% sodium azide.
Extra Details:: 8-hydroxyguanine, a form of oxidative DNA damage induced by free radicals, causes G:C to T:A transversion. In E. coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine. One of these enzymes, MutM, was found to have a functional yeast (yOgg1) and human (hOgg1) homologue. hOgg1 proteins efficiently released the 8-hydroxyguanine opposite the pyrimidine from DNA and cleaved the AP site in a manner similar to bacterial and yeast enzymes. Genetic backgrounds in control of the repair of damaged DNA are involved in the susceptibility to cancer development. The hOgg1 gene has been mapped to region 3p26.2, a region showing loss of heterozygosity (LOH) in a variety of cancers. In particular, 3p25-p26 is a common LOH region in lung cancer. Recent work has demonstrated that Ogg plays an important role in CAG expansion, a characteristic of several neurodegenerative diseases. Ogg appears to be responsible for progressive expansion of poly-Q tracts in response to oxidative damage. Thus, Ogg provides a direct link between DNA damage and toxicity in neurons. Applications: Suitable for use in ELISA, Immunohistochemistry, Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 1:1000 Immunohistochemistry (Frozen): 1:25-1:100 Western Blot: 1:500-1:1000. Recognizes a band at ~39kD in human sample. Immunofluorescence (IC): 1:100-1:200 Optimal dilutions to be determined by the researcher. Positive Control: Jurkat whole cell extracts Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice