O5403-01B

OCT-4 (POU5F1, POU domain, class 5, transcription factor 1, NF-A3, Oct3, Oct-3, Oct3/4, Oct-3/4, Octamer-binding transcription factor 3, Otf3, Otf-3, Otf3g, Otf3-rs7, Otf4, Otf-4)

Size

£877.00

SKU:: O5403-01B
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa1-145 from human Oct-4. Cellular Localization: Nucleus.
Clone:: 7F9.2
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis, expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known. Applications: Suitable for use in Immunocytochemistry, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 1:100-1: 200 Western Blot: 1-2ug/ml Immunocytochemistry: 1:100-1: 200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice