Oct-1 (Organic Cation Transporter 1)

Size

£767.00

SKU:: O5400-06
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide consisting of 21aa within the cytoplasmic, C-terminus of rat Oct1 (KLH). Species Sequence Homology: Mouse: 90%; human: 66%; rabbit: 64%.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Extra Details:: Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called "renal secretion". Functional studies have identified two distinct categories of organic cation transporters (Octs): a system driven by transmembrane potential difference that governs the influx of cations, whereas the H+-gradient- dependent transport system may mediate the efflux of organic cations. Several multispecific, potential-sensitive transporters (Oct1-3) and H+-dependent transporters (OctN1-3) have been cloned and characterized from various tissues. Oct superfamily of proteins share a high degree of sequence homology and display up to 12 transmembrane domains with cytoplasmic N- and C-terminus. Oct1 (rat/mouse 556aa, ~95% homology; human 554aa, ~78% homology with rat Oct1) is expressed primarily in the kidney, liver and intestine. Rat Oct1 has been localized to the basolateral membrane of small intestinal enterocytes, hepatocytes, and S1 segment of the proximal renal tubules. Oct1 mediated uptake of TEA was pH and Na+-independent and was reduced when membrane potential decreased. Oct1 also transported NMN, choline, MPP, dopamine, thiamine, noradrenaline, histamine, and spermine but not for putrescine. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml. Band at ~66kD (rat kidney). Optimal dilutions to be determined by the researcher. Control Peptide: O5400-05: Oct-1, Rat, Control Peptide Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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