O5204
Occludin
Size
£709.00
- SKU:
- O5204
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Fusion protein containing the C-terminal 150aa of human occludin.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.1-1ug/ml Western Blot: 1-3ug/ml Immunofluorescence: 4-20ug/ml Immunoprecipitation: ~5ug/IP reaction Immunohistochemistry (FFPE): 2-5ug/ml; Protease pretreatment of sections is required prior to staining. Dilute protease to 1-2mg/ml and incubate at 37°C for 10 minutes. Other enzyme and HIER pretreatment methods may not be suitable for this antibody. Optimal dilutions to be determined by the researcher. Recommended IF fixation and staining protocol: 1.“Pre-extract” the cells on ice for 2 min with an ice-cold buffer containing 0.2% Triton X-100. 2. Fix cells in 3% paraformaldehyde in PBS for 30 min at RT. 3. Permeabilize sample with 0.05% Triton X-100 in PBS for 5 min on ice. 4. Incubate samples with the desired concentration of O5204 (4-20ug/ml) on ice for 1 hour. 5. Incubate with IgG (FITC) Pab Gt x Rb for 30 min at RT. Recommended Secondary Antibodies: I1904-40D: IgG, H&L (FITC) Pab Gt xRb I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb I1904-46N: IgG, H&L (FITC) Pab Gt xRb Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice