O5204-HRP
Occludin (HRP)
Size
£965.00
- SKU:
- O5204-HRP
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Fusion protein containing the C-terminal 150aa of human occludin.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (FFPE): Protease pretreatment of sections is required prior to staining. Dilute protease to 1-2mg/ml and incubate at 37°C for 10 minutes. Other enzyme and HIER pretreatment methods may not be suitable for this antibody. Optimal dilutions to be determined by the researcher. Recommended IF fixation and staining protocol: 1.“Pre-extract” the cells on ice for 2 min with an ice-cold buffer containing 0.2% Triton X-100. 2. Fix cells in 3% paraformaldehyde in PBS for 30 min at RT. 3. Permeabilize sample with 0.05% Triton X-100 in PBS for 5 min on ice. 4. Incubate samples with the desired concentration of O5204 (4-20ug/ml) on ice for 1 hour. 5. Incubate with IgG (FITC) Pab Gt x Rb for 30 min at RT. Recommended Secondary Antibodies: I1904-40D: IgG, H&L (FITC) Pab Gt xRb I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb I1904-46N: IgG, H&L (FITC) Pab Gt xRb Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice