O5200
Occludin (HRP)
Size
£787.00
- SKU:
- O5200
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- GST fusion protein consisting of the C-terminal region of human occludin
- Clone:
- 3G217
- Format:
- Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.19% Kathon™ CG, 40% glycerol. Labeled with Horseradish peroxidase (HRP).
- Extra Details:
- The 65kD occludin protein was first identified in chicken using monoclonal antibodies(11,12). The chicken occludin cDNA was subsequently cloned and sequenced, and the amino acid sequence revealed that the occludin protein is organized into five distinct domains: a short amino terminal cytoplasmic domain (domain A), two extracellular loops (domains B and D) separated by a short intracellular loop (domain C), and a long carboxy-terminal cytoplasmic tail (domain E)(11,12). The C-terminal tail of occludin is required for both for its localization at tight junctions and for its direct interaction with the ZO-1 protein(12). One interesting feature of the occludin protein is that its amino acid sequence has not been highly conserved throughout evolution(13). This fact made isolating the mammalian homologues of chicken occludin a rather difficult task. Recently, however, the sequences of the full length cDNAs encoding occludin of rat-kangaroo, human, mouse, and dog were reported(13). At the amino acid level, the human, murine, and canine occludin proteins are highly homologous (~90% identity); however, the mammalian proteins exhibit a considerable degree of divergence from the rat kangaroo and chicken proteins(13). Applications: Suitable for use in ELISA, Western Blot.. Other applications not tested. Recommended Dilutions: ELISA: 0.1-1ug/ml Western Blot: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma) and rat liver. Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice