Organic Anion Transporter 3 (OAT3)

Size

£735.00

SKU:: O3003-20
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 16aa near the N-terminus, cytoplasmic domain of rat OAT3, conjugated to KLH. Species Sequence Homology: mouse, 93%; human and rabbit, 87%.
Format:: Supplied as liquid in PBS, 0.1% BSA.
Extra Details:: Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues. OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Recently OAT3 (rat 536aa, human 568aa) has been cloned that is most closely related to OAT1 (~49% identity). Rat OAT3 is expressed in the liver, brain, kidney and eye. OAT3 also mediates Na-independent uptake of several organic anions (PAH, ochratoxin, and estrone sulfate, etc). OAT3 shows 92% homology with mouse Roct (reduced in osteosclerosis transporter), possibly an ortholog of rat OAT3. As compared to OAT1-2, strong expression of OAT3 in brain suggests its role in removing organic anion in the brain. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml. Coat with ELISA plates with Control Peptide at 1ug/ml. Immunohistochemistry: 2-20ug/ml Immunofluorescence: 2-20ug/ml Optimal dilutions to be determined by the researcher. Control Peptide: O3003-22 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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