O3003-11
Organic Anion Transporter 2 (OAT2)
Size
£754.00
- SKU:
- O3003-11
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A 15aa peptide sequence near the extracellular domain, N-terminus of rat OAT2 (KLH).
- Format:
- Supplied as liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
- Extra Details:
- Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues. OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). OAT2, previously called NLT (novel liver-specific transporter) shows 42% homology with OAT2. OAT2 (rat 535 aa; human 548 aa) is predominantly expressed in liver, and to a lower level in kidney. OAT2 mediates sodium-independent, multispecific organic anion transport (PAH, salicylate and acetylsalicylate, prostaglandin E2, and dicarboxylate). Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml using ECL. ELISA: 0.5-1ug/ml. Optimal dilutions to be determined by the researcher. Recommended Control Peptide: O3003-12. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice