Organic Anion Transporter 1 (OAT1)

Size

£754.00

SKU:: O3003-02
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A synthetic peptide corrsponding to 16aa near the cytoplasmic, C-terminus of rat OAT1, conjugated to KLH. Species Sequence Homology: mous and, rabbit; 87%, human; 81%.
Format:: Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Extra Details:: Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues. OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Rat Kidney PAH transporter, termed OAT1 or ROAT1, encodes a protein of 551 aa. Human OAT1 (hOAT1) is 563 aa and its alternatively spliced isoform hOAT2 is 550 aa (missing 13 aa from 523-533 aa). OAT1 has wide substrate selectivity, covering endogenous substrates such as cyclic nucleotides, prostaglandin and uric acid, and a variety of drugs (e.g., antibiotics, non-steroidal anti-inflammatory drugs, diuretics, anti-neoplastic drug, and a uricosuric drug). OAT1 has been localized on the basolateral membrane of the proximal tubule in the kidney. Weak expression was also detected in brain. OAT1 has ~95 identity with mouse NKT, an ortholog of rat OAT1. Applications: Suitable for use in ELISA Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1-10ug/ml using ECL technique. ELISA: 0.5-1ug/ml Immunohistochemistry: 1-20ug/ml Immunofluorescence: 1-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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