O3001-35
OATP2, 8 (Organic Anion Transporting Polypeptide 2, 8)
Size
£793.00
- SKU:
- O3001-35
- Purity:
- Hybridoma culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to 24aa from the N-terminus of human OATP2 conjugated to KLH.
- Clone:
- 5F260
- Format:
- Supplied as a liquid, 0.09% sodium azide.
- Extra Details:
- Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. The OATP family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Oatp2 (also called OATP-B1; 661 aa; 12 TM), cloned from rat brain, shares significant homology with rat oatp1 and OAT-K1 (77%) and human OATP (73%). It is highly expressed in brain, liver, and kidney but not in heart, spleen, lung, muscle, and testes. OATP2 mediates uptake of taurocholate, estrogen conjugates, and cardiac glycosides ouabain. Unlike other OATPS, OATP2 also transport digioxin. Applications: Suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin sections): Requires microwave pretreatment. Incubate overnight at 4°C. Immunofluorescence: Stains the sinusoidal (basolateral) membranes of human liver and hepatocelular carcinoma. Incubate for 1 hour at RT. Western Blot: For optimal detection of the glycosylated antigen, sample boiling before SDS-PAGE is not recommended. Alternatively, samples can be kept at 37°C for 30 minutes. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice