OATP2 (Organic Anion Transporting Polypeptide 2)

Size

£754.00

SKU:: O3001-09
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthertic peptide corresponding to 12aa near the C-terminus of rat OATP2, conjugated to KLH. Species Sequence Homology: mouse; 50%
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, 0.1% BSA Reconstitute with 100ul sterile PBS.
Extra Details:: Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs and other organic anions from the blood by a process called “renal secretion.” Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway and placenta. Several multispecific OATs (OAT1-3, OAT-K1, OATK2) and OATPs (oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). OAT2, previously called NLT (novel liver-specific transporter) shows 42% homology with OAT2. OAT2 (rat 535 aa; human 548 aa) is predominantly expressed in liver, and to a lower level in kidney. OAT2 mediates sodium-independent, multispecific organic anion transport (PAH, salicylate and acetylsalicylate, prostaglandin E2, and dicarboxylate). Applications: Suitable for use in ELISA and Immunohistochemistry. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. . Recommended Dilutions: ELISA: 0.5-1ug/ml Immunohistochemistry (Paraformaldehyde fixed): 2-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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