N8590

NY-ESO-1 (Cancer/Testis Antigen 1, Autoimmunogenic Cancer/Testis Antigen NY-ESO-1, Cancer/Testis Antigen 6.1, CT6.1, L Antigen Family Member 2, LAGE-2)

Size

£934.00

SKU:: N8590
Application:: WB
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: NY-ESO-1 full-length recombinant protein
Clone:: 4H362 (E978)
Format:: Supplied as a liquid, 0.05% sodium azide
Extra Details:: Human gene NY-ESO-1 codes for antigens which are expressed in malignancies of various histological types but not in normal tissues except testis. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:5000-1:20,000 detects NY-ESO-1 protein in RIPA lysates from SKMEL-19 cells. SKMEL-19 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with N8590 (1:20,000). Proteins were visualized using an IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system. Optimal dilution to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.