N6939-69B
Nucleolar Protein, 30kD (NO30, Nop30)
Size
£655.00
- SKU:
- N6939-69B
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- 14 amino acid peptide from near the carboxy terminus of human Nop30 (GenBank accession no. AAH12798)
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation (1 for review). Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and the caspase family of proteases. CARD domain containing cell death regulators include RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD (3). An alternate splicing isoform of ARC was identified as Nop30 (3). While ARC interacts with caspase-2 and –8 and suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1 and DR3 (2), Nop30 multimerizes and binds to the splicing factor SRp30c and may act to influence alternative splice site selection in vivo (3). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml. See N6939-69C for control peptide. Optimal dilutions to be determined by the researcher. Positive Control: Mouse skeletal tissue lysate Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice