N6925
Nuclei
Size
£902.00
- SKU:
- N6925
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human nuclei from cell extracts
- Clone:
- 2Q2234 (235-1)
- Format:
- Supplied as a liquid in PBS. No preservative added.
- Extra Details:
- Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:10-1:250, 4% PFA fixed cryosections, or 5-10 minuted fixed cell cultures. Acetone fixation is also effective. Permeabilization with 0.1-0.4% triton X-100 is suggested in the blocking buffer. Immunohistochemistry (Formalin fixed, paraffin embedded tissues): 1:20 for 1hr at RT. Citrate buffer epitope retrieval recommended. Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice