NR0B1 (Nuclear Receptor Subfamily 0 Group B Member 1, Nuclear Receptor DAX-1, DSS-AHC Critical Region On The X Chromosome Protein 1, DAX1, AHC, DAX1)

Size

£642.00

SKU:: N6889-92D
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to CGEDHPQQGSTLY, from the internal region of human NROB1.
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: NROB1 contains a DNA-binding domain and acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in the gene for NROB1 result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. Applications: Suitable for use in Peptide ELISA, Flow Cytometry, Immunofluorescence and Western Blot Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:32,000 Western Blot: 0.5-2ug/ml, ~55kD band observed in human adrenal gland lysates Flow Cytometry: 10ug/ml Immunofluorescence: 10ug/ml. Strong expression of the protein seen in the nuclei and cytoplasm of HeLa and U2OS cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.