N6888-85

Nuclear Pore Complex, nup153 (NPC)

Size

£793.00

SKU:: N6888-85
Purity:: Supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Nuclear matrix protein fraction prepared from rat liver nuclei.
Clone:: 5F280
Format:: Supplied as a liquid, 0.09% sodium azide.
Extra Details:: Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence microscopy and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:5-1:10; Incubation time 1 hr at RT (frozen sections; also after fixation with paraformaldehyde). Western Blot: 1:30-1:50 (ECL method). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.