N5396-11A
NSF (N-ethylmaleimide-sensitive Factor)
Size
£581.00
- SKU:
- N5396-11A
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues surrounding Leu524 of human NSF.
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Extra Details:
- Several protein-protein interactions are essential to membrane fusion during endocytosis. Interactions among SNARE1 proteins associated with donor membranes and acceptor membranes are required for fusion (1,2). After membrane fusion, alpha-SNAP, a cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then can associate with the alpha-SNAP/SNARE complex and mediate SNARE diassembly during the membrane fusion process (3,4). The ATPase activity of NSF induces a conformational change in the alpha-SNAP/SNARE complex, leading to its dissociation from the membrane and membrane fusion as well as recycling of the SNARE complex for subsequent rounds of fusion (3,4). Applications: Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice