NR1, phosphorylated (Ser897) (N-methyl-D-aspartate Receptor subunit NR1, GRIN1, NMDA1, NMDAR1, NMD-R1, Glutamate receptor ionotropic N-methyl D-aspartate 1) (Biotin)

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£1,196.00

SKU:: N5380-11-BIOTIN
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to rat NR1 phosphorylated at Ser897, conjugated to KLH. Species Sequence Homology: mouse and human, 100%
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml detected NR1 in 10ug of rat brain microsomal tissue lysate. Immunohistochemistry: 1:50 detected NR1 in normal rat hippocampus. Immunoprecipitation: 10ug from a previous lot precipitated NR1 from rat brain microsomal RIPA lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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