N5380-05-PE

NR1, CT (NMDA (N-methyl-D-aspartate) Receptor subunit NR1) (PE)

Size

£1,190.00

SKU:: N5380-05-PE
Purity:: Purified by Protein G affinity chromatography from ascites
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: His tag fusion protein corresponding to aa834-938 of rat NR1. Peptide sequence homology: human and mouse, 100%
Clone:: 0.T.190
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Extra Details:: NR1 along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory. Applications: Suitable for use in Immunocytochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: Do not boil the microsomal preparation. Incubate at RT for 30-45 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Brain tissue Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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