N5380-05-BIOTIN
NR1, CT (NMDA (N-methyl-D-aspartate) Receptor subunit NR1) (Biotin)
Size
£1,190.00
- SKU:
- N5380-05-BIOTIN
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- His tag fusion protein corresponding to aa834-938 of rat NR1. Peptide sequence homology: human and mouse, 100%
- Clone:
- 0.T.190
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
- Extra Details:
- NR1 along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory. Applications: Suitable for use in Immunocytochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: Do not boil the microsomal preparation. Incubate at RT for 30-45 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Brain tissue Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice