N5379-60H

NQO2, Recombinant, Human (NAD(P)H Dehydrogenase Quinone 2, DIA6, DHQV, Ribosyldihydronicotinamide Dehydrogenase [Quinone], NRH Dehydrogenase [Quinone] 2, NRH:Quinone Oxidoreductase 2, NMOR2, Quinone Reductase 2, QR2)

Size

£320.00

SKU:: N5379-60H
Application:: WB
Molecular Weight:: 28.1
Purity:: ~ 95% (SDS-PAGE). Purified by using conventional chromatography techniques.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 10% glycerol.
Extra Details:: NQO2 is a member of the NAD(P)H dehydrogenase (quinone). The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones. This detoxification process protects cells against quinone-induced oxidative stress, cytotoxicity and mutagenicity. Recombinant human NQO2 protein was expressed in E. coli. Recombinant corresponding to aa1-231 of human NQO2 with a 6x His tagged expressed in E. coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MAGKKVLIVY AHQEPKSFNG SLKNVAVDEL SRQGCTVTVS DLYAMNFEPR ATDKDITGTL SNPEVFNYGV ETHEAYKQRS LASDITDEQK KVREADLVIF QFPLYWFSVP AILKGWMDRV LCQGFAFDIP GFYDSGLLQG KLALLSVTTG GTAEMYTKTG VNGDSRYFLW PLQHGTLHFC GFKVLAPQIS FAPEIASEEE RKGMVAAWSQ RLQTIWKEEP IPCTAHWHFG Q Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice