NPTXR (Neuronal Pentraxin Receptor, NPR)

Size

£908.00

SKU:: N5379-08R
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Ala24-Lys499 of human NPTXR, expressed in NS0 cells. Species Sequence Homology: mouse (87%)
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: NPTXR (Neuronal Pentraxin Receptor; also NPR) is a 65kD, type II transmembrane glycoprotein that binds Neuronal Pentraxins 1 and 2. It is expressed on cerebellar Purkinje and granule cells, and hippocampal neurons of the CA1, CA3 and dentate gyrus regions. Human NPTXR is 499 amino acids (aa) in length. It contains a two aa cytoplasmic tail, a 21aa transmembrane segment, and a 476aa extracellular region with one Pentraxin domain (aa295-499). One alternate start site exists at Met12, and a 55kD soluble form (possibly beginning at Gln56) may be generated by proteolytic cleavage. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human brain (hippocampus) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice