N5376-85B

Noxa (Phorbol-12-Myristate-13-Acetate-Induced Protein 1, PMA-induced Protein 1, Immediate-early-response Protein APR, Protein Noxa, Pmaip1) (Biotin)

Size

£761.00

SKU:: N5376-85B
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: GST-fusion protein corresponding to human Noxa.
Clone:: 11E299 (114C307.1)
Format:: Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Biotin.
Extra Details:: Recently, a BH-3 only member of the Bcl-2 family have been identified in human and mouse and designated as Noxa (for damage). The expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Noxa cDNA codes for a 103aa protein. The coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as, Bcl-XL and Mcl-1. Oda et al have also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treatment of cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis suggesting that Noxa may represent a mediator of p53-dependent apoptosis. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice