Noxa (Adult T cell Leukemia-derived PMA Responsive, APR, Immediate Early Response Protein APR, PMAIP1, PMA-induced Protein 1, Phorbol-12-myristate-13-acetate-induced Protein 1) (Not for Export EU)

Size

£735.00

SKU:: N5376-80D
Application:: WB
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide (MPGRKARRNA PVNPTR) as part of mouse Noxa (aa1-16) conjugated to diphtheria toxoid.
Format:: Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O.
Extra Details:: The Bcl-2 family of proteins which regulate apoptosis share identical sequences called Bcl-2 Homology domains (BH1-4). The BH3 proteins, including BID, NOXA, PUMA, BIK, BIM and BAD are all pro-apoptotic and share sequence identity within the amphipathic alpha-helical BH3 region, which is essential for their apoptotic function. NOXA is highly expressed in adult T-cell leukemia cell line. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.