N5376-78

NOX5 (NADPH Oxidase 5, NADPH Oxidase, EF-hand Calcium Binding Domain 5, MGC149776, MGC149777, NOX5A, NOX5B)

Size

£642.00

SKU:: N5376-78
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-EWHPFTISSAPEQKD, from human NOX5, at the internal region of the protein (NP_078781.3, NP_001171708.1, NP_001171709.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:8000 Western Blot: 0.5-2ug/ml, human testis lysates observed on ~90kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.