Notch 2 (Notch2, Notch-2, Aicardi-Goutieres Syndrome 2, AGS2, Drosophila Notch Homolog 2, hN2, N2, Neurogenic Locus Notch Homolog Protein 2 Precursor, Notch (Drosophila) Homolog 2, Notch Homolog 2 (Drosophila), Notch Homolog 2)

Size

£735.00

SKU:: N5375-15E
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide representing the C terminal region of the human NOTCH homolog 2 (NOTCH2) protein.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.