N5375-03Z
Notch-1 (Notch1, Neurogenic Locus Notch Homolog Protein 1, Notch 1, hN1, Translocation-associated Notch Protein TAN-1, Notch 1 Extracellular Truncation, Notch 1 Intracellular Domain, TAN1) (Biotin)
Size
£984.00
- SKU:
- N5375-03Z
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- NS0-derived, recombinant protein corresponding to Ala19-Gln526 from human Notch-1.
- Clone:
- 10K83
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Labeled with Biotin. Reconstitute with sterile 500ul PBS. Labeled with Biotin.
- Extra Details:
- Notch-1 (so named for "notches" in fly wings; also TAN-1) is a 300kD, 2538aa member of the notch family of type I TM glycoproteins. It is associated with gene activation in both embryo and adult. It undergoes Golgi processing to generate a heterodimer composed of a 180-200kD disulfide-linked extracellular domain (aa18-1664), and a 120kD membrane-bound segment (aa1665-2556) that is cleaved to form the notch intracellular domain transcription factor upon ligand binding. Human Notch-1 ECD aa19-526, including the first 13 EGF repeats, shows 91% aa identity with corresponding regions of mouse and rat Notch-1, and 60% aa identity with human Notch-2 and Notch-3. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 0.5-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice