N5375-03N
Notch-1 (9930111A19RIK, hN1, LIN12, Lin-12, MIS6, Neurogenic Locus Notch Homolog Protein 1, NOTC1, Notch (Drosophila) Homolog 1 (Translocation Associated), Notch 1 Extracellular Truncation, Notch 1 Intracellular Domain, Notch Homolog 1, Notch Homolog 1 Translocation Associated (Drosophila), Translocation Associated (Drosophila), Translocation Associated Notch Homolog, Translocation Associated Notch Protein TAN1)
Size
£787.00
- SKU:
- N5375-03N
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- PA317-derived, recombinant mouse Notch-1.
- Clone:
- 9j15
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Notch-1 (so named for "notches" in fly wings; also TAN-1) is a 300kD, 2538aa member of the notch family of type I TM glycoproteins. It is associated with gene activation in both embryo and adult. It undergoes Golgi processing to generate a heterodimer composed of a 180-200kD disulfide-linked extracellular domain, and a 120kD membrane-bound segment that is cleaved to form the notch intracellular domain transcription factor upon ligand binding. Mouse Notch-1 ECD (aa19-526), including the first 13 EGF repeats, shares 91% and 95% aa identity with corresponding regions of human and rat Notch-1, respectively, and 55-58% aa identity with mouse Notch-2 and Notch-3. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice