N5375-03L
Notch-1 (hN1, LIN12, Lin-12, MIS6, Neurogenic Locus Notch Homolog Protein 1, NOTC1, Notch (Drosophila) Homolog 1 (Translocation Associated), Notch 1 Extracellular Truncation, Notch 1 Intracellular Domain, Notch Homolog 1, Notch Homolog 1 Translocation Associated (Drosophila), Translocation Associated (Drosophila), Translocation Associated Notch Homolog, Translocation Associated Notch Protein TAN1) (APC)
Size
£863.00
- SKU:
- N5375-03L
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa19-526 from human Notch-1 expressed in NS0 cells.
- Clone:
- 9H167
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- Notch-1 (so named for “notches” in fly wings; also TAN-1) is a 300kD, 2538aa member of the Notch family of type I TM glycoproteins. It is associated with gene activation in both embryos and adults. It undergoes Golgi processing to generate a heterodimer composed of a 180-200 kD disulfide-linked extracellular domain (aa18-1664), and a 120kD membrane-bound segment (aa1665-2556) that is cleaved to form the notch intracellular domain transcription factor upon ligand binding. Human Notch-1 ECD (aa19-526), including the first 13 EGF repeats, shows 91% aa identity with corresponding regions of mouse and rat Notch-1, and 60% aa identity with human Notch-2 and Notch-3. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells in U2OS human cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice