NOS III (Endothelial Nitric Oxide Synthase, eNOS, NOS 3)

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£754.00

SKU:: N5350-28B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: 15aa peptide sequence corresponding to human eNOS. (KLH)
Format:: Supplied as a liquid in PBS, pH 7.5, 0.1% BSA, 40% glycerol.
Extra Details:: Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitters, NO is not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues. NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-III, MW ~130-160kD). One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principal group is inducible, Ca2+/calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II). Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible. Sequence homology among different cloned isoforms is ~ 50%. Human, rat, mouse and bovine eNOS/NOS-3 are ~1202-1205aa proteins. Applications: Suitable for use in ELISA, Western Blot and Immunocytochemistry, and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: Coat plates with control peptide (Cat# N5350-28A) at 1ug/ml. Western Blot: 1-10ug/ml; Recognizes 135-140kD protein. Immunocytochemistry/Immunohistochemistry: 5-10ug/ml. Useful on tissue sections fixed with 3.5% paraformaldehyde. We recommend overnight incubation with antibody at 4°C and detection by ABC (peroxidase) technique. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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