NOS I (NOS1, nitric oxide synthase 1 (neuronal), NOS, neuronal nitric oxide synthase, PnNOS, penile neuronal nitric oxide synthase, penile neuronal NOS)

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£642.00

SKU:: N5350-01E
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: C-ESKKDTDEVFSS, C Terminus
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Alternate Name: NOS1, nitric oxide synthase 1 (neuronal), NOS, neuronal nitric oxide synthase, PnNOS, penile neuronal nitric oxide synthase, penile neuronal NOS Domain Information: PDZ domain (Also known as DHR or GLGF); Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]; pfam02898, NO_synthase, Nitric oxide synthase, oxygenase domain Gene Ontology Terms: nitric oxide synthase activity; calmodulin binding; electron transporter activity; nitric oxide biosynthesis; electron transport; synaptic transmission; muscle contraction; intracellular signaling cascade; cell-cell signaling; membrane fraction; oxidoreductase activity Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Western blot: Approx 160kD and 45kD bands seen in Mouse Brain extracts. Recommended for use at 0.5-2ug/ml. Staining (0.5ug/ml) of Mouse Brain extracts (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See N5350-01E-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

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