N4000-80
Norrin (Norrie Disease Protein)
Size
£812.00
- SKU:
- N4000-80
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Lys25-Ser133 of human Norrin expressed in E. coli.
- Clone:
- 7H103
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Norrin is a secreted protein that has a C-terminal cysteine-knot motif. Although Norrin is unrelated to Wnt family proteins, it is a Frizzled-4-specific ligand that activates the canonical Wnt pathway through Frizzled-4 and LRP5 (LDL receptor-related protein). Mutations in Norrin are associated with Norrie disease and X-linked familial exudative vitroretinopathy. The amino acid sequence of human Norrin is 100% and 94% identical to rhesus macaque and mouse Norrin, respectively. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml under reducing conditions only. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice