Nocodazole Solution in DMSO, 5mg/ml

Size

£133.00

SKU:: N3000-01
Molecular Weight:: 301.32
Storage Conditions:: 4°C
Supplier:: United States Biological
CAS Number:: 31430-18-9
Extra Details:: Nocodazole, an inhibitor of microtubule formation, was found to greatly inhibit cell division around the mound stage as well as during the vegetative growth phase, when applied to exponentially growing Ax-2 cells. Mitotic arrest of cells with nocodazole can be used to study phosphorylation of microtubule-associated proteins which increase during mitosis, concomitant with increased microtubule dynamics. Nocodazole has been found to dramatically inhibit T cell antigen receptor (TCR) signaling, independently of its ability to depolymerize microtubules. Nocodazole arrests the cell cycle at G2/M phase and prevents phosphorylation of the T cell antigen receptor and inhibits its activity. Synonyms: Methyl N-(5-thenoyl-2-benzimidazolyl)carbamate; Methyl [5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl]carbamate; Oncodazole; R 17934; [5-(2-Thienylcarbonyl)-1H-benzimidazol-2-yl]-carbamic acid methyl ester CAS No (dry powder): 31430-18-9 Molecular Formula: C14H11N3O3S Molecular Weight (dry powder): 301.32 Source: Synthetic Purity: Nocodazole: ≥99% (HPLC); DMSO: ≥99.9% Appearance: Supplied as a liquid in DMSO. Method for Determining Identity: Proton NMR Spectroscopic Analysis Concentration: 5mg/ml Usage: Use stock solution at 5mg/ml in DMSO. Further dilutions can be made in culture media. Due to insolubility (nocodazole is only slightly soluble in water), no more than 10ug/ml concentration can be used in cell culture systems. Nocodazole is stable in biological media for seven days. Storage and Stability: May be stored at RT for short-term only. Long-term storage is recommended at 4°C for 6 months after receipt.

Shipping Conditions:: Ambient