N2920-05
NNA1 (Cytosolic Carboxypeptidase 1, ATP/GTP-binding Protein 1, Nervous System Nuclear Protein Induced by Axotomy Protein 1 Homolog, CCP1, KIAA1035, AGTPBP1)
Size
£642.00
- SKU:
- N2920-05
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Synthetic peptide corresponding to C-TSPLEYNLPS, from human AGTPBP1, at the internal region of the protein (NP_056054.2).
- Format:
- Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
- Extra Details:
- Nervous system nuclear protein induced by axotomy 1 (Nna1, AGTPBP1 or cytosolic carboxypeptidase-1) is related to zinc carboxypeptidases and contains an ATP/GTP binding motif, a basic and a bipartite nuclear localization signal. Nna1 expression is rapidly induced in motor neurons following axotomy and down-regulated following reinnervation, which is consistent with abundant Nna1 expression in differentiating neurons and the absence of Nna1 in CNS proliferative zones. Furthermore, Purkinje cell degeneration is characterized by adult onset neurodegeneration resulting from Nna1 gene mutations, implicating Nna1 in mechanisms common to degeneration and regeneration. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:8000 Western Blot: 1-3ug/ml, lysates of cell line HeLa observed on ~150kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice